JOURNAL OF PHARMACOLOGY AND
BIOMEDICINE
ISSN.2456-8244
A new RP-HPLC method was developed for the estimation of Rizatriptan in tablets and it was validated as per ICH guide- lines. The chromatogram for was found to be satisfactory on sym- metry C-18 (4.6×150mm, 5µ Thermosil column) using mobile phase composed of 60:40%v/v phosphate buffer of pH 3.6 and iso- propyl alcohol at a flow rate of 1.0 ml/min. The retention time of Rizatriptan was found to be 7.924 min. The system suitability pa- rameters proved that the proposed method is suitable for estima- tion of Rizatriptan. Tailing factor for the peak was found to be 1.22 and the theoretical plates for separation were found to be 3563. The method was found to be linear in the range of 10-50µg/ml. The precision of the method was good and the recovery of drugs is well within the acceptance limits of 80-120%. The LOD and LOQ were found to be 0.024µg/ml and 0.08 µg/ml respectively. The proposed RP HPLC method was found suitable for the estimation of Rizatriptan in formulations and is simple, selective, reproduci- ble and accurate with good precision and can be successfully ap- plied to routine analytical purpose.
NAMES:
ONLINE ISSN:2456-8244
Keywords: HPLC Rizatriptan Validation ICH Dosage form
DOI:
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