The objective of the present study was to assess the antioxidant potential of different leaf extract of Petunia hybrida using the in vitro model. The extraction ability of different solvents for recovering extractable components from leaves followed the order: ethanol>chloroform>aqueous>pet ether. The findings of preliminary phytochemical analysis suggest the presence of alkaloids, saponin glycosides, phenolics, terpenoids, sterols, and flavonoids in the leaf of the plant. The total phenolic content of pet ether, chloroform, ethanol and aqueous extracts of Petunia hybrida were 16.18±0.89, 23.97±0.61, 71.77±0.59 and 31.38±0.36 GAE mg/g, respectively. All the extracts were subjected to in vitro determination of antioxidant potential by DPPH method. The IC50 value of the DPPH scavenging potential for the chloroform, ethanol and aqueous extracts was found to be 163.3, 96.1 and 153.2 µg/mL respectively. The reducing potential was found to be dose dependent and the followed the order ethanolic>aqueous>chloroform. The IC50 value of the percent HRSA for the chloroform, ethanol and aqueous extracts was found to be 225.90, 172.02, and 177.7 µg/mL respectively. The IC50 value of the percent PRP for the chloroform, ethanol and aqueous extracts was found to be 226.17, 172.72, and 223.14 µg/mL respectively. It was found that the chloroform fraction was best able to inhibit the phosphomolybdenum complex.

Articles Details

NAMES:

ONLINE ISSN:2456-8244

Keywords: Petunia hybrida, DPPH, antioxidant, phosphomolybdenum, extract, hydroxyl, scavenging

DOI:

Abstract FullText PDF

REFERENCES

1. Ansari AQ, Ahmed SA, Waheed MA, Sayyed JA. Extraction and determination of antioxidant activity of Withania somnifera Dunal. Eur J Experimental Biol 2013, 3(5): 502-507
2. Griesbach RJ, Asen S, Leonnarat BA. Petunia hybrida anthocyanins acylated with caffeic acid. Phytochemistry. 1991; 30(5): 1729-1731
3. https://en.wikipedia.org/wiki/Petunia_%C3%97_atkinsiana; assessed on 19/07/2021
4. Kalita JC, Milligan SR. In vitro estrogenic potency of phytoestrogen-glycosides and some plant flavanoids. Indian Journal of Science and Techology. 2010; 3(12): 1142-1147
5. Sas K, Robotka H, Toldi J, Vecsei L. Mitochondrial, metabolic disturbances, oxidative stress and kynurenine system, with focus on neurodegenerative disorders. J Neurol Sci 2007, 257: 221–239.
6. Schram AW, Jonsson LMV, de Vlaming P. Identification of Anthocyanins and Intermediates of Anthocyanin Biosynthesis from Petunia hybrida Using High Performance Liquid Chromatography. Z Naturforsch. 1983; 38c: 342-345
7. Thenmozhi M, Sivaraj R. In Vitro evaluation of the antibacterial activity of Petunia leaf and callus extracts. Journal of Agriculture Techology. 2011; 7(2): 321-330
8. Vogel GH (Ed). Endorinology. In: Drug Discovery and Evaluation: Pharmacological Assays. Springer-Verlag, Berlin, Heidelberg; 2002