The primary objective of the current investigation was to standardize the formulation Glucomap Tablet, marketed as an effective treatment for management of diabetes. The procured tablets of GMT were brownish green in color, with no odor and a bitter taste. They were available as rough textured tablets with no coating, stored free from moisture. GMT exhibited 8.3% total ash with 2.05% acid insoluble ash and 5.75% water soluble ash. The water soluble and alcohol soluble extractives were 1.14% and 1.22% respectively suggesting the formulation to be suitable for human use. The results of preliminary qualitative phytochemical screening revealed that the classes of phytochemicals were present in GMT. TLC analysis of GMT was done using charantin as the marker using methanol: benzene (2:8) as the solvent system. Charantin appeared at Rf value of 0.56 on the TLC plate. The quantitation of the charantin was done by HPLC method and it was found that GMT contained 8.6 mg charantin per 500 mg of GMT (1.72 %). The antidiabetic potential of the GMT tablet was determined by extracting the tablets with ethanol and administering at a dose of 200mg/kg to alloxan induced diabetic rats. GMTEE was able to decrease blood sugar by 23.62% while the standard drug glibenclamide could reduce it by 28.12% on the 15th day.

Articles Details

NAMES:

ONLINE ISSN:2456-8244

Keywords: Standardization, herbal, glucomap, charantin, antidiabetic, WHO

DOI:

Abstract FullText PDF

REFERENCES

1. Anonymous. Quality Control Methods for Medicinal Plant Materials. World Health Organisation. Geneva, (1998) 25-28
2. Chandana M. Puttaraju, Manorama Eti. WHO 75 gram OGTT-A single step procedure for screening and diagnosis of gestational diabetes mellitus. Int J Reproduction Contraception Obstetrics and Gynecology. 2015, 4(6). DOI: http://dx.doi.org/10.18203/2320-1770.ijrcog20151307
3. https://maharishiayurvedaindia.com/products/glucomap; assessed on 13/01/2021
4. Mehta S, Singh RP, Saklani P. Phytochemical screening and TLC profiling of various extracts of Reinwardtia indica. Int J Pharmacogn Phytochem Res, 2017; 9(4): 523-527.
5. Patel S, Patel T, Parmar K, Bhatt Y, Patel Y, Patel NM. Isolation, characterization and antimicrobial activity of charantin from Momordica charantia Linn. fruit, Int J Drug Dev Res, 2010; 2(3): 629-634.
6. Szkudelski, T., 2001. The mechanism of alloxan and streptozotocin action in B-cells of the rat pancreas. Physiol. Res. 50, 536.
7. Zold EL, Horvath A, Bacsadi B, Csupor D. Development and validation of a HPLC-UV method for quantification of charantin in momordica charantia products. 2019; DOI: 10.14232/tnpr.2019.af